similar results  (ATCC)

Journal: bioRxiv

Article Title: Image Correlation Spectroscopy is a Robust Tool to Quantify Cellular DNA Damage Response

doi: 10.1101/2024.08.05.606697

Figure Lengend Snippet: ( A ) Schematic population of monomeric fluorescent particles where emitting particles (green) are in a diffraction limited excitation focus (cyan) – not to scale. ( B ) Schematic showing increase in particle density from A) which results in an increase in average fluorescence intensity. ( C ) Schematic showing clustering of fluorescent particles where the total number of independent particle clusters is the same as A). Spatial ICS measures the average number of independent fluorescent units per area which, along with the average fluorescence intensity, is used to calculate the average degree of aggregation of the particles. ( D ) Intensity-based segmentation of the DAPI channel with an automatic threshold using the MATLAB function imbinarize to create a mask of the nucelus. The mask specifies the ROI for ICS analysis of the γH2AX channel, outputting mean number of independent fluorescent particles per focal spot area (inverse of the intensity normalized spatial correlation function amplitude). ( E ) and ( F ) Comparison of the ICS parameter TNoP with E) ImageJ Find Maxima tool with a user defined threshold and F) Imaris Spots with an automatic threshold for the same nuclei. A linear line of best fit is shown on each graph with the resulting R 2 .

Article Snippet: Overall, in OVCA429, ICS DA produced similar results to Imaris Spots in olaparib treated cells, but measured dose dependent response in cells treated with veliparib where Imaris Spots was unable to capture the dose dependency.

Techniques: Fluorescence, Comparison

Journal: bioRxiv

Article Title: Image Correlation Spectroscopy is a Robust Tool to Quantify Cellular DNA Damage Response

doi: 10.1101/2024.08.05.606697

Figure Lengend Snippet: ( A-C ) SKOV3 cells treated with 0.1 mM MMS; A) DAPI channel with 10 µm scale bar, B) γH2AX channel, C) γH2AX channel with intensity-adjustment. ( D-L ) Data for normalized intensity, normalized number of spots and normalized ICS DA of γH2AX signal across different MMS ( D-F ), veliparib ( G-I ) and olaparib ( J-L ) treatment concentrations in SKOV3 cells. Shown are individual cells, average for each biological repeat (n=3, squares), and average with standard deviation. Results are normalized to average DMSO. MMS; N=2423-3160, veliparib; N=1818-2922, olaparib; N=1489-2956. * indicates p<0.05, ** indicates p<0.001, *** indicates p<1X10 −10 via one-way ANOVA significance testing.

Article Snippet: Overall, in OVCA429, ICS DA produced similar results to Imaris Spots in olaparib treated cells, but measured dose dependent response in cells treated with veliparib where Imaris Spots was unable to capture the dose dependency.

Techniques: Standard Deviation

Journal: bioRxiv

Article Title: Image Correlation Spectroscopy is a Robust Tool to Quantify Cellular DNA Damage Response

doi: 10.1101/2024.08.05.606697

Figure Lengend Snippet: A-C ) OVCA429 cells treated with 0.1 mM MMS; A) DAPI channel with 10 µm scale bar, B) γH2AX channel, C) γH2AX channel with intensity-adjustment. ( D-L ) Data for normalized intensity, normalized number of spots and normalized ICS DA of γH2AX signal across different MMS ( D-F ), veliparib ( G-I ) and olaparib ( J-L ) treatment concentrations in OVCA429 cells. Shown are individual cells, average for each biological repeat (n=3, squares), and average with standard deviation. Results are normalized to average DMSO. MMS; N=1194-2068, veliparib; N=1256-1997, olaparib; N=1445-2334. * indicates p<0.05, ** indicates p<0.001, *** indicates p<1X10 −10 via one-way ANOVA significance testing.

Article Snippet: Overall, in OVCA429, ICS DA produced similar results to Imaris Spots in olaparib treated cells, but measured dose dependent response in cells treated with veliparib where Imaris Spots was unable to capture the dose dependency.

Techniques: Standard Deviation

Journal: bioRxiv

Article Title: Image Correlation Spectroscopy is a Robust Tool to Quantify Cellular DNA Damage Response

doi: 10.1101/2024.08.05.606697

Figure Lengend Snippet: ( A-C ) Control SKOV3 cells, ( D-F ) SKOV3 cells treated with 1 mM MMS; ( A&D ) DAPI channel with 10 µm scale bar, ( B&E ) RPA1 channel, ( C&F ) RPA1 channel with intensity-adjustment. Normalized RPA1 Imaris Spots ( G ) and normalized ICS DA ( H ) in SKOV3 and OVCA429 cells ( I&J ). Shown are individual cells, average for each biological repeat (n=3, squares), and average with standard deviation. Results are normalized to average DMSO. N=1600-1872 for SKOV3 and N=1320-2198 for OVCA429. ( K-M ) Control SKOV3 cells, ( N-P ) SKOV3 cells treated with 1 mM MMS; ( K&N ) DAPI channel with 10 µm scale bar, ( L&O ) RAD51 channel, ( M&P ) RAD51 channel with intensity-adjustment. Normalized RAD51 Imaris Spots ( Q ) and normalized ICS DA ( R ) in SKOV3 and OVCA429 cells ( S&T ). Shown are individual cells, average for each biological repeat (n=3, squares), and average with standard deviation. Results are normalized to average DMSO. N=1597-2241 for SKOV3 and N=1822-2311 for OVCA429. All results, * indicates p<0.05, ** indicates p<0.001, *** indicates p<1X10 −10 via one-way ANOVA significance testing.

Article Snippet: Overall, in OVCA429, ICS DA produced similar results to Imaris Spots in olaparib treated cells, but measured dose dependent response in cells treated with veliparib where Imaris Spots was unable to capture the dose dependency.

Techniques: Control, Standard Deviation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Insights into the l , d -Transpeptidases and d , d -Carboxypeptidase of Mycobacterium abscessus : Ceftaroline, Imipenem, and Novel Diazabicyclooctane Inhibitors

doi: 10.1128/AAC.00098-20

Figure Lengend Snippet: MICs for 20 Mycobacterium abscessus clinical strains and the reference strain ATCC 19977 of ceftaroline and imipenem with and without relebactam (REL) and avibactam (AVI)

Article Snippet: Similar MIC results were observed with the M. abscessus type strain, ATCC 19977. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Drug MIC range (μg/ml) MIC 50 (μg/ml) MIC 90 (μg/ml) CEF 2–32 8 32 Imipenem 0.5–2 1 2 CEF + avibactam (4 μg/ml) a 0.25–2 0.5 1 Imipenem + CEF (1 μg/ml) <0.001–2 0.25 0.5 Imipenem + CEF (1 μg/ml) + REL (4 μg/ml) <0.01–2 0.25 0.5 Open in a separate window a MICs for 21 isolates only.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Insights into the l , d -Transpeptidases and d , d -Carboxypeptidase of Mycobacterium abscessus : Ceftaroline, Imipenem, and Novel Diazabicyclooctane Inhibitors

doi: 10.1128/AAC.00098-20

Figure Lengend Snippet: MIC 50 s and MIC 90 s for 55 M. abscessus clinical strains of ceftaroline, imipenem, and ceftaroline (CEF)-imipenem with and without relebactam (REL)

Article Snippet: Similar MIC results were observed with the M. abscessus type strain, ATCC 19977. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Drug MIC range (μg/ml) MIC 50 (μg/ml) MIC 90 (μg/ml) CEF 2–32 8 32 Imipenem 0.5–2 1 2 CEF + avibactam (4 μg/ml) a 0.25–2 0.5 1 Imipenem + CEF (1 μg/ml) <0.001–2 0.25 0.5 Imipenem + CEF (1 μg/ml) + REL (4 μg/ml) <0.01–2 0.25 0.5 Open in a separate window a MICs for 21 isolates only.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Insights into the l , d -Transpeptidases and d , d -Carboxypeptidase of Mycobacterium abscessus : Ceftaroline, Imipenem, and Novel Diazabicyclooctane Inhibitors

doi: 10.1128/AAC.00098-20

Figure Lengend Snippet: MIC distributions of imipenem (IMI) (a), ceftaroline (CEF) (b), imipenem with 1 μg/ml ceftaroline (c), and imipenem with 1 μg/ml ceftaroline and 4 μg/ml relebactam (REL) (d) against 55 M. abscessus clinical strains. The MIC data are presented in Table S1.

Article Snippet: Similar MIC results were observed with the M. abscessus type strain, ATCC 19977. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Drug MIC range (μg/ml) MIC 50 (μg/ml) MIC 90 (μg/ml) CEF 2–32 8 32 Imipenem 0.5–2 1 2 CEF + avibactam (4 μg/ml) a 0.25–2 0.5 1 Imipenem + CEF (1 μg/ml) <0.001–2 0.25 0.5 Imipenem + CEF (1 μg/ml) + REL (4 μg/ml) <0.01–2 0.25 0.5 Open in a separate window a MICs for 21 isolates only.

Techniques: